Journal: Cell Death & Disease

Article Title: Gadd45a opens up the promoter regions of miR-295 facilitating pluripotency induction

doi: 10.1038/cddis.2017.497

Figure Lengend Snippet: Gadd45a upregulates miR-290 clusters in reprogramming. ( a ) Phase contrast and fluorescence photographs of MEFs infected with SKO plus Flag or Gadd45a, SKOM plus Flag or Gadd45a. Flag is the empty vector control. Arrows point to emerging colonies. ( b ) Gadd45a significantly enhances iPSC generation in various mediums. In the absence of vitamin C, the numbers of GFP+ colonies were counted at day 17; in the presence of vitamin C, the numbers of GFP+ colonies were counted at day 13; in iCD1 medium, the numbers of GFP+ colonies were counted at day 7. Data are represented as mean±S.D.; n =3. * P ≤0.05, ** P ≤0.01, *** P ≤0.001. ( c ) Gadd45a overexpression blocks the formation of partially reprogrammed cells. The colonies were stained for alkaline phosphatase (AP). The numbers of AP+GFP+ colonies and AP+GFP− colonies were counted. Data are represented as mean±S.D. n =3; *** P ≤0.001. ( d ) Heatmaps depicting the relative fold change of miRNA expression at day 8 post infection by miRNA microarray. Red and green colors indicated increased and decreased expression, respectively. ( e ) qPCR analysis of miR-294 and miR-295 expression level during SKO- and SKOM-mediated reprogramming in the presence and absence of Gadd45a. Data are represented as mean±S.D.; n =3. *** P ≤0.001

Article Snippet: Real-time RT-PCR was performed with the All-in-One miRNA qRT-PCR Detection Kit (GeneCopoeia, Rockville, MD, USA) following the manufacturer’s instructions.

Techniques: Fluorescence, Infection, Plasmid Preparation, Control, Over Expression, Staining, Expressing, Microarray

Journal: Frontiers in Immunology

Article Title: Experimental Demyelination and Axonal Loss Are Reduced in MicroRNA-146a Deficient Mice

doi: 10.3389/fimmu.2018.00490

Figure Lengend Snippet: Differential expression of microRNAs (miRNAs) in the corpus callosum of mice during experimental demyelination and remyelination. Using microarray and validation by quantitative PCR (qPCR), three miRNAs, miR-146a (A) , miR-193a (B) and miR-181b (C) were differentially regulated in response to CPZ exposure in the corpus callosum. * p < 0.05, ** p < 0.01, *** p < 0.001, n = 5–8 in each group, one-way ANOVA, mean ± SEM. Abbreviation: C: un-manipulated controls covering ages of mice used in the de- and remyelinating experiments, 4wD: 4 weeks demyelination. 2dR: 2 days (acute) remyelination, 2wR: 2 weeks (full) remyelination.

Article Snippet: For microRNA profiling, the Agilent Mouse miRNA Microarray Kit (G4472A, 8 × 15k) was applied according to the manufacturer’s instruction (version 1.0) with 100–100 ng quality-checked total RNA.

Techniques: Expressing, Microarray, Real-time Polymerase Chain Reaction

Journal: Frontiers in Immunology

Article Title: Experimental Demyelination and Axonal Loss Are Reduced in MicroRNA-146a Deficient Mice

doi: 10.3389/fimmu.2018.00490

Figure Lengend Snippet: Expression of experimentally validated miR-146a target genes and proteins in response to CPZ exposure in wild-type and miR-146-deficient mice. (A) Expression of miR-146a target genes (miRTarBase) among 1,239 differentially expressed genes in the corpus callosum in response to CPZ exposure determined by a 4 × 44K Agilent Whole Mouse Genome Microarray (NCBI Gene Expression Omnibus with accession GSE100663). (B) The protein expression of validated miR-146a target genes SMAD4 and SNAP25 were downregulated in the corpus callosum of miR-146a KO mice compared to WT mice by liquid chromatography mass spectrometry in response to CPZ. (C) SNAP25 and (D) SMAD4 in corpus callosum lysates of miR-146a KO and WT mice in response to CPZ examined by ELISA. * p < 0.05, ** p < 0.01, *** p < 0.001, n = 8–4 in each group, mean ± SEM. Abbreviations: C: un-manipulated controls, 4wD: 4 weeks demyelination. 2dR: 2 days (acute) remyelination, 2wR: 2 weeks (full) remyelination.

Article Snippet: For microRNA profiling, the Agilent Mouse miRNA Microarray Kit (G4472A, 8 × 15k) was applied according to the manufacturer’s instruction (version 1.0) with 100–100 ng quality-checked total RNA.

Techniques: Expressing, Microarray, Liquid Chromatography, Mass Spectrometry, Enzyme-linked Immunosorbent Assay

Journal: Journal of Clinical Laboratory Analysis

Article Title: Candidate Biomarkers Associated With Circulating Tumor Cell Status in Metastatic Colorectal Cancer

doi: 10.1002/jcla.70013

Figure Lengend Snippet: The expression profiles of DEM groups with RNA‐seq data in COAD and READ datasets.

Article Snippet: Clinical information for the entire sample is provided in Table . cDNA synthesis was carried out using the miRCURY LNA miRNA RT Kit (QIAGEN GmbH, Hilden, Germany) on the SimpliAmp Thermal Cycle (Applied Biosystems, Foster City, CA), following the manufacturer's protocol.

Techniques: Expressing

Journal: Journal of Clinical Laboratory Analysis

Article Title: Candidate Biomarkers Associated With Circulating Tumor Cell Status in Metastatic Colorectal Cancer

doi: 10.1002/jcla.70013

Figure Lengend Snippet: Normalized qPCR expression of selected significantly downregulated miRNAs and their ROC curve analysis (A) hsa‐miR‐199a‐5p (B) hsa‐miR‐326 (C) hsa‐miR‐500b‐5p ( p < 0.05, AUC > 0.7).

Article Snippet: Clinical information for the entire sample is provided in Table . cDNA synthesis was carried out using the miRCURY LNA miRNA RT Kit (QIAGEN GmbH, Hilden, Germany) on the SimpliAmp Thermal Cycle (Applied Biosystems, Foster City, CA), following the manufacturer's protocol.

Techniques: Expressing

Journal: Journal of Clinical Laboratory Analysis

Article Title: Candidate Biomarkers Associated With Circulating Tumor Cell Status in Metastatic Colorectal Cancer

doi: 10.1002/jcla.70013

Figure Lengend Snippet: Comparison of microarray and qPCR results for selected three miRNAs in CTC(+)/CTC(−), CTC(−)/HIs, and mCRC/HIs groups.

Article Snippet: Clinical information for the entire sample is provided in Table . cDNA synthesis was carried out using the miRCURY LNA miRNA RT Kit (QIAGEN GmbH, Hilden, Germany) on the SimpliAmp Thermal Cycle (Applied Biosystems, Foster City, CA), following the manufacturer's protocol.

Techniques: Comparison, Microarray

Journal: Journal of Clinical Laboratory Analysis

Article Title: Candidate Biomarkers Associated With Circulating Tumor Cell Status in Metastatic Colorectal Cancer

doi: 10.1002/jcla.70013

Figure Lengend Snippet: Comparison of logFC values for the selected three miRNAs across different datasets: Microarray and qPCR analyses compare HIs and mCRC groups, while TCGA‐COAD and TCGA‐READ datasets compare HIs and CRC groups (incl. mCRC).

Article Snippet: Clinical information for the entire sample is provided in Table . cDNA synthesis was carried out using the miRCURY LNA miRNA RT Kit (QIAGEN GmbH, Hilden, Germany) on the SimpliAmp Thermal Cycle (Applied Biosystems, Foster City, CA), following the manufacturer's protocol.

Techniques: Comparison, Microarray